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KMID : 0861020020170020183
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Korea Journal of Herbology
2002 Volume.17 No. 2 p.183 ~ p.190
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Inhibition effect of water extract of Cnidii Rhizoma on Angiotensin 2-induced angiogenesis
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Abstract
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Objective : To estimate the angiogenic effect of water extract of Cnidii Rhizoma (ECR), we employed the rat sponge model for angiogenesis.
Methods : It was based on subcutaneous implantation of sterile polyester sponges in the SD rat and we have characterized the dye method that incorporates 5% carmine red and 1% cresyl violet in 10% gelatin. vascularity index (V.I.) expressed as ㎍ dye/mg tissue. Subcutaneous implantation of sterile polyester sponges in SD rats enhanced the neovascularization over 14 days, as determined by measurements of relative sponge blood flow by a vascularity technique. The angiogenic response was verified by histological evaluation of vascularized sponges.
Results : Daily oral administration of ECR (100 mg/kg for 14 days) produce no apparent effect on angiogenesis. The vasoconstrictor peptide, angiotensin Ⅱ (AⅡ), in daily doses of 100 nmol, caused an intense neovascularization but lower doses of AⅡ produced no apparent effect (1 nmol and 10 nmol). Co-administration of 100 nmol AⅡ and 100 mg/kg ECR p.o. depressed 100 nmol AⅡ-induced angiogenic effect.
Conclusion : We have shown that p.o. daministration of 100 nmol AⅡ accelerated angiogenesis. Meanwhile, co-administration of 100 nmol AⅡ and 100 mg/kg ECR highly suppressed angiogenic property.
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